Fig. 3.

Non-cytotoxic doses of CPC inhibit RBL-2H3 mast cell degranulation. (A) RBL-2H3 mast cells were sensitized with IgE and stimulated to degranulate with 0.0004 μg/mL Ag for 1 h, ± CPC in BT; the spontaneous (“spont.”) response was determined by cells which were not exposed to Ag or CPC. Normalized degranulation response is plotted against CPC exposure concentrations. Effects of CPC on RBL-2H3 cell survivability were assessed by LDH assay (B) and by trypan blue-exclusion (C). Values presented are means ± SEM of three independent experiments; three replicates were used in each experiment. Statistical significance, compared to the Control (0 μM CPC), is represented by **p < 0.01, ***p < 0.001 and determined by one-way ANOVA followed by Tukey's post-test. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)