Fig. 5.

CPC affects PIP₂-binding protein cluster properties in NIH-3T3 cells. Two-color TIRF FPALM was used to obtain images from fixed NIH-3T3 cells co-expressing HA-Dendra2 and PAmKate-PH. NIH-3T3 cells were exposed to Control (0 μM CPC), 5 μM, and 10 μM CPC for 1 h at 37 °C, then were fixed with 4% PFA. PH domain cluster properties (A) Mean density of a PH domain cluster, (B) Mean number of PH domain molecules forming a cluster, (C) Mean Area of a PH domain cluster, (D) Mean perimeter of a PH domain cluster for Control, 5 μM, and 10 μM CPC-treated cells were quantified using SLCA. Values represent mean ± SEM of three independent experiments from analysis of n = 25 cells for BT Control (0 μM CPC), n = 26 cells for 5 μM CPC, and n = 29 cells for 10 μM CPC. Statistically significant results are represented by **p < 0.01, as compared to the Control by one-way ANOVA followed by Dunnett's multiple comparison test against the Control.