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. 2022 Jan;11(1):91–103. doi: 10.21037/tau-21-1110

Figure 4.

Figure 4

MT suppresses the transcriptional activity of AR. (A) 293T cells were co-transfected with AR expressing vector, ARE-luciferase reporter construct, and pRL-TK (internal control) for 24 h, and then treated with MT at the indicated doses for an additional 48 h. The transfected cells were harvested and assayed for luciferase activity. (B) RT-qPCR analysis of AR and (C) PSA transcript level in LNCaP cells treated with MT (2 mM) for 48 h. (D) Western blot analysis of AR and PSA protein expression in LNCaP cells treated with MT (2 mM) for 48 h. Data in (A-C) are presented as means ± SEM. of three independent experiments. *P<0.05, **P<0.01 versus control group. MT, melatonin; AR, androgen receptor; ARE, AR-response elements; PSA, prostate-specific antigen; LNCaP, Lymph Node Carcinoma of the Prostate.