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. 2022 Jan 1;13(3):858–868. doi: 10.7150/jca.60730

Figure 2.

Figure 2

Knockdown of FOXK2 suppressed the proliferation of PTC cells. (A) Western blot detection of FOXK2 expression in human normal thyroid follicular epithelial cells and papillary thyroid cancer cell lines. Tubulin was used as a loading control. (B) The protein expression of FOXK2 in human normal thyroid follicular epithelial cells and papillary thyroid cancer cell lines was visualized via quantitative analysis. ** P < 0.01; ***P < 0.001. (C) Western blot detection of FOXK2 in BHT-101 cells transfected with indicated plasmids. (D) The protein expression of FOXK2 in BHT-101 cells was visualized via quantitative analysis. ***P < 0.001. (E) Western blot detection of FOXK2 in BCPAP cells transfected with indicated plasmids. (F) The protein expression of FOXK2 in BCPAP cells was visualized via quantitative analysis. ** P < 0.01. (G, H) The ability of cell proliferation was monitored by daily quantification in BHT-101 and BCPAP cell lines transfected with indicated plasmids for up to 4 days. ** P < 0.01; ***P < 0.001. (I, J) The ability of cell colony formation was detected in BHT-101 and BCPAP cell lines transfected with indicated plasmids. ***P < 0.001.