Knockdown of FOXK2 promoted the expression of autophagy-related protein ULK1, VPS34 and FOXO3. (A) Western blot detection of ULK1, VPS34 and FOXO3 in BHT-101 cells transfected with indicated plasmids. (B-D) The protein expression of ULK1 (B), VPS34 (C) and FOXO3 (D) in BHT-101 cells was visualized via quantitative analysis. * P < 0.05. (E) Western blot detection of ULK1, VPS34 and FOXO3 in BCPAP cells transfected with indicated plasmids. (F-H) The protein expression of ULK1 (F), VPS34 (G) and FOXO3 (H) in BCPAP cells was visualized via quantitative analysis. ** P < 0.01. (I) Western blot detection of FOXK2, ULK1, VPS34, FOXO3 and p62 in BCPAP cells transfected with indicated plasmids and siRNA.