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. 2022 Jan 1;13(3):858–868. doi: 10.7150/jca.60730

Figure 5.

Figure 5

FOXK2 knockdown inhibited the proliferation of PTC cells through its regulating autophagy. (A) Western blot detection of p62 and LC3 in BHT-101 cells transfected with indicated plasmids with or without 3-MA. Tubulin was used as a loading control. (B) The protein expression of p62 in BHT-101 cells treated with 3-MA was visualized via quantitative analysis. ** P < 0.01. (C) The protein expression of LC3II/I in BHT-101 cells treated with 3-MA was visualized via quantitative analysis. *P < 0.05. (D) The ability of cell proliferation was monitored by daily quantification in BHT-101 cells transfected with indicated plasmids for up to 4 days with or without 3-MA. ** P < 0.01. (E-F) The ability of cell colony formation was detected in BHT-101 cell lines transfected with indicated plasmids with or without 3-MA. * P < 0.05; **P < 0.01.