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. 2022 Jan 1;13(3):764–774. doi: 10.7150/jca.63490

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Non-absorbable antibiotic treatment inhibits tumor liver metastasis in mouse models. (A) Flowchart of the antibiotic treatment model. (B) HE staining of mouse liver tissues and analysis of mouse blood ALT levels revealed no significant changes after two weeks of antibiotic treatment. Sections were observed under a microscope at 400×. (C) Liver metastasis in BALB/c mice after spleen injection of 1.5×10⁶ CT26luc cells. (D) Metastasis indexes and (E) luminescence intensities showed that antibiotic treatment significantly reduced liver metastasis in BALB/c mice. (F) Liver metastasis in C57BL/6J mice after spleen injection of 2×10⁶ MC38 cells. (G) Metastasis indexes showed that antibiotic treatment significantly decreased liver metastasis in C57BL/6J mice. N.S., no significance; *P < 0.05, **P < 0.01.