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. 2021 Dec 1;188(2):1095–1110. doi: 10.1093/plphys/kiab558

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© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Auxin biosynthesis is required for BBM-mediated somatic embryogenesis. 35S:BBM-GR seeds were grown for 14 d in the presence of DEX and imaged at the indicated time points. The YUC enzyme inhibitor yucasin (100 µM) was added or removed during the culture period as indicated (day +y or day −y). A–E, DEX-treated samples to which YUC inhibitor was added on Day 0, 2, 4, 6, 8, or 10. F, DEX-treated control sample. G–J, DEX-treated samples in which YUC inhibitor was added on Day 0 and then removed on Day 4, 6, 8, or 10. Scale bars, 1 mm.