Figure 8.

Auxin biosynthesis is required to maintain BBM-induced totipotency. Confocal laser scanning micrographs of cotyledon/cotyledon tips of 35S:BBM-GR explants grown with DEX, with or without the YUC enzyme inhibitor YDF (100 µM). A, Control DEX-treated explants. B–D, Explants treated with DEX and YDF, which was added or removed on the days indicated by gray blocks in each row. Samples were counter stained with SR2200 (magenta). The day of culture is indicated in the panels. Yellow arrowheads, WOX2 expression (yellow signal). Asterisks, autofluorescence. Scale bars, 100 μm.