Figure 2.

Expression of MdCHS3 in poplar xylem tissue is associated with accumulation of naringenin in hydrolyzed methanolic extract. A, Relative expression levels of MdCHS3 in transgenic poplar xylem tissue determined by RT-qPCR gene expression analysis are shown as fold change (FC) relative to the lowest expressing tree, originating from MdCHS3-poplar line 6. Expression of MdCHS3 was not detectable in WT poplar. Expression levels represent the mean across five biological replicates, standard error indicated by error bars. B, Naringenin released after hydrolysis of methanolic extracts of MdCHS3-poplar xylem tissue. No naringenin was detectable in WT extracts (detectable levels >0.70 $\mu$g/g xylem tissue). Values represent the mean across five biological replicates for lines 5, 14, 7, and 2. Naringenin was detectable in only four of five biological replicates in line 6 (n = 4). Standard error indicated by error bars.