Table 1.
Cell wall composition of WT and MdCHS3-poplar xylem
| Lignin content (mg/100 mg) |
Monomer composition |
Percentage of Holocellulose | Percentage of Alpha cellulose | |||
|---|---|---|---|---|---|---|
| Poplar line | Acid-soluble lignin | Acid-insoluble lignin | Total lignin | S:G ratio | ||
| WT | 3.47 (0.23) | 16.72 (0.12) | 20.19 (0.30) | 2.79 (0.05) | 63.77 (0.62) | 35.90 (0.36) |
| Line 6 | 3.56 (0.12) | 16.04 (0.36) | 19.60 (0.38) | 2.71 (0.10) | 66.75 (1.00) | 37.20 (1.39) |
| Line 5 | 4.01 (0.15) | 16.15 (0.19) | 20.16 (0.20) | 2.71 (0.09) | 65.42 (0.69) | 33.02 (1.53) |
| Line 14 | 3.35 (0.10) | 15.70 (0.17) | 19.05 (0.22) | 2.74 (0.02) | 66.74 (0.98) | 37.53 (1.30) |
| Line 7 | 3.22 (0.21) | 14.94 (0.39) | 18.16 (0.47) | 2.77 (0.03) | 64.70 (0.76) | 36.24 (0.60) |
| Line 2 | 3.42 (0.12) | 14.87 (0.27) | 18.27 (0.30) | 2.65 (0.05) | 64.50 (0.29) | 38.77 (1.06) |
Total lignin content measured in extract-free whole-cell wall material of WT control and MdCHS3-hybrid poplar xylem determined using Klason lignin analysis. Lignin monomeric composition was determined by thioacidolysis and relative % carbohydrate cell wall content was determined by holocellulose and alpha cellulose reactions. Values represent the mean across five biological replicates per line, standard error in brackets. Statistically significant differences compared to WT are bolded (P < 0.05) and were determined using Student’s t test.