Figure 4.

Immunoblotting analysis of mitochondrial dynamics proteins. (A) Representative immunoblots for control and mAPP-HT22 cells with or without mitophagy enhancers. (B) Quantitative densitometry analysis for mitochondrial dynamics proteins—significantly increased levels of fission proteins Drp1 and Fis1 were observed in cells transfected with mutant APP. Fusion proteins Mfn1, Mfn2 and Opa1 were significantly decreased. On the other hand, mitophagy enhancers treated mutant APP showed reduced levels of fission proteins and increased levels of fusion proteins were observed.