Table 2.
Summary of qRT-PCR oligonucleotide primers used in measuring mRNA expressions in mitochondrial dynamics and mitochondrial biogenesis, synaptic and mitophagy genes in mitophagy enhancers treated and untreated mutant APP-HT22 cells
| Gene | DNA sequence (5′-3′) | PCR product size |
|---|---|---|
| Mitochondrial dynamics genes | ||
| Drp1 | Forward Primer ATGCCAGCAAGTCCACAGAA | 86 |
| Reverse Primer TGTTCTCGGGCAGACAGTTT | ||
| Fis1 | Forward Primer CAAAGAGGAACAGCGGGACT | 95 |
| Reverse Primer ACAGCCCTCGCACATACTTT | ||
| Mfn1 | Forward Primer GCAGACAGCACATGGAGAGA | 83 |
| Reverse Primer GATCCGATTCCGAGCTTCCG | ||
| Mfn2 | Forward Primer TGCACCGCCATATAGAGGAAG | 78 |
| Reverse Primer TCTGCAGTGAACTGGCAATG | ||
| Opa1 | Forward Primer ACCTTGCCAGTTTAGCTCCC | 82 |
| Reverse Primer TTGGGACCTGCAGTGAAGAA | ||
| Mitochondrial biogenesis genes | ||
| PGC1α | Forward Primer GCAGTCGCAACATGCTCAAG | 83 |
| Reverse Primer GGGAACCCTTGGGGTCATTT | ||
| Nrf1 | Forward Primer AGAAACGGAAACGGCCTCAT | 96 |
| Reverse Primer CATCCAACGTGGCTCTGAGT | ||
| Nrf2 | Forward Primer ATGGAGCAAGTTTGGCAGGA | 96 |
| Reverse Primer GCTGGGAACAGCGGTAGTAT | ||
| TFAM | Forward Primer TCCACAGAACAGCTACCCAA | 84 |
| Reverse Primer CCACAGGGCTGCAATTTTCC | ||
| Reverse Primer AGACGGTTGTTGATTAGGCGT | ||
| Synaptic genes | ||
| Synaptophysin | Forward Primer CTGCGTTAAAGGGGGCACTA | 81 |
| Reverse Primer ACAGCCACGGTGACAAAGAA | ||
| PSD95 | Forward Primer CTTCATCCTTGCTGGGGGTC | 90 |
| Reverse Primer TTGCGGAGGTCAACACCATT | ||
| Mitophagy genes | ||
| Pink1 | Forward Primer CCATCGGGATCTCAAGTCCG | 70 |
| Reverse Primer GATCACTAGCCAGGGACAGC | ||
| Parkin | Forward Primer AGAGGTCCAGTTAAACCCACC | 90 |
| Reverse Primer GAGGGTTGCTTGTTTGCAGG | ||
| Housekeeping genes | ||
| B-actin | Forward Primer AGAAGCTGTGCTATGTTGCTCTA | 91 |
| Reverse Primer TCAGGCAGCTCATAGCTCTTC | ||