Table 3.
Summary of antibody dilutions and conditions used in the immunoblotting analysis of mitochondrial dynamics, mitochondrial biogenesis, synaptic and mitophagy proteins in mitophagy enhancers treated and untreated mAPP-HT22 cells and untransfected HT22 cells
| Marker Primary antibody—species and dilution | Purchased from company, city and state | Secondary antibody, dilution | Purchased from company, city and state |
|---|---|---|---|
| 6E10 Mouse monoclonal 1:500 | Biolegend, San Diego, CA | Sheep anti-mouse HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |
| Drp1 Rabbit polyclonal 1:500 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |
| Fis1 Rabbit polyclonal 1:500 | Protein Tech Group, Inc., Chicago, IL | Donkey anti-rabbit HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |
| Mfn1 Rabbit polyclonal 1:400 | Abcam, Cambridge, MA | Donkey anti-rabbit HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |
| Mfn2 Rabbit polyclonal 1:400 | Abcam, Cambridge, MA | Donkey anti-rabbit HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |
| OPA1 Rabbit polyclonal 1:500 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |
| SYN Rabbit monoclonal 1:400 | Abcam, Cambridge, MA | Donkey anti-rabbit HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |
| PSD95 Rabbit monoclonal 1:300 | Abcam, Cambridge, MA | Donkey anti-rabbit HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |
| PGC1a Rabbit polyclonal 1:500 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |
| NRF1 Rabbit polyclonal 1:300 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |
| NRF2 Rabbit polyclonal 1:300 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |
| TFAM Rabbit polyclonal 1:300 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |
| PINK1 Rabbit polyclonal 1:500 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |
| Parkin Mouse polyclonal 1:500 | Novus Biological, Littleton, CO | Sheep anti-mouse HRP 1:10000 | GE Healthcare Amersham, Piscataway, NJ |