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. 2021 Nov 15;188(2):1189–1209. doi: 10.1093/plphys/kiab531

Figure 1.

Figure 1

Gene silencing of 35S::OsGA2ox1. A, Morphology of 110-d-old TP309, dwarf GAE, semi-dwarf GAE, and GAS plants. Scale bar = 10 cm. B, Genotyping transgenic OsGA2ox1. OsActin served as the control. GAE, OsGA2ox1ectopic expression; GAS, OsGA2ox1silencing. C, The mRNA level of OsGA2ox1 as indicated by semi-quantitative RT-PCR. OsActin served as the control. D, Western blot detection of the protein level of SLR1. E, Chop-PCR assay on the gene body of OsGA2ox1 in the indicated genotypes. F, sRNA northern blot detection of the sRNA levels on the gene body of OsGA2ox1. G, Chop-PCR assay of the 35S promoter in the indicated genotypes.