Figure 9.

Expression levels of RdDM genes are upregulated in reproductive tissues. A, Heatmap showing the relative expression levels of DNA methylation-related genes in various tissues from the Rice XPro database (https://ricexpro.dna.affrc.go.jp/GGEP/index.php). B, Western blot detection of the protein levels of OsRDR2 in various tissues of two independent OsRDR2::OsRDR2-3xFLAG transgenic rice plants. C, DNA methylation-related genes overlapped with panicle-, stamen-, and pistil-CHH hyper-DMRs. D, Percentages of DNA methylation-related genes overlapped with panicle CHH hyper-DMRs (left), stamen CHH hyper-DMRs (middle), and pistil CHH hyper-DMRs (right) (^**P < 0.01; by Fisher’s two-sided test). E, Integrated Genome Browser view of CHH methylation levels on OsNRPD1b in various organs of the indicated genotypes. F, Heatmap showing the expression levels of DNA methylation-related genes in WT seedlings, WT pistils, and osrdr2–3 pistils.