Skip to main content
. 2021 Sep 7;31(3):376–385. doi: 10.1093/hmg/ddab254

Figure 2.

Figure 2

Loss of CL does not cause a generic decline in uniporter subunits. (A) SDS-PAGE immunoblot analysis of MICU1 in mitochondria isolated from WT and crd1Δ yeast expressing human MICU1. Por1 is used as a loading control. (B) Quantification of relative MICU1 levels from (A) using ImageJ software. Data shown as mean ± SD. n = 3. ****P < 0.0001. (C) SDS-PAGE immunoblot of MCUR1-V5 in mitochondria isolated from WT and crd1Δ yeast expressing human MCUR1 tagged with V5. Por1 is used as a loading control. (D) Quantification of relative MCUR1 levels from (C) using ImageJ software. Data shown as mean ± SD. n = 3. ns, not significant. (E) BN-PAGE immunoblot of EMRE in mitochondria isolated from C2C12 WT and Taz-KO cells. Blot is representative of three independent experiments. (F) BN-PAGE immunoblot of MCUR1 in mitochondria isolated from C2C12 WT and Taz-KO cells. Blot is representative of three independent experiments WT, wild type.