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MCU-mediated mitochondrial Ca2+ uptake is reduced in C2C12 Taz-KO cells. (A–D) Permeabilized C2C12 WT and Taz-KO cells were pulsed with different [Ca2+] as indicated. Representative traces show bath [Ca2+] (μM). (E) Rate of [Ca2+]m as function of MCU channel activity. Data are represented as mean ± SEM. n = 3. *P < 0.05, ns, not significant. [Ca2+]m, mitochondrial Ca2+.
