Figure S3.

Flow cytometric gating strategies, related to Figures 1 and 3

(A) Gating strategy in this study used to distinguish antigen-specific, class-switched B cells.

(B) Gating strategy in this study used to distinguish bona fide pulmonary tissue-resident memory CD8⁺ (top) or CD4⁺ (bottom) T cells.

(C) Gating strategy in this study used to distinguish neutrophils, alveolar macrophages (AMs), and interstitial macrophages (IMs) from other major pulmonary myeloid cell populations.

Examples shown are representative from BALB/c mice i.n. vaccinated with Tri:ChAd at 4 weeks post-immunization.