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. 2021 Sep 7;31(3):386–398. doi: 10.1093/hmg/ddab255

Figure 2.

Figure 2

Inducible targeting of PYL at the GFP reporter. (A) Schematic representation of 16B-Y (left) and 91B-Y (right) cell lines. (B) ChIP-qPCR using antibodies against FLAG to pull down PYL at INT and ACTA1 in 16B-Y cells (left, N = 4) and 91B-Y cells (right, N = 4) after 5-day treatments with ABA or DMSO. The error bars represent the standard error. (C) Representative flow cytometry profiles after a 5-day treatment as well as quantification of the GFP expression in 16B-Y (left, N = 6) and 91B-Y (right, N = 6) cells. The error bars represent the standard deviation around the mean. (D) Representative SP-PCR blots after 30 days of continuous culture in the presence of DMSO (left) or ABA (right) in 91B-Y cells. One nanogram of DNA/reaction used in both cases.