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. 2021 Aug 26;188(2):683–702. doi: 10.1093/plphys/kiab349

Figure 1.

Figure 1

LSFM of transgenic Arabidopsis plants carrying genetically encoded molecular markers, which are developmentally and subcellularly regulated during root hair development. A and B, ANNEXIN1-GFP (ANN1-GFP) as a cytosolic marker. C and D, GFP-ROOT HAIR DEFECTIVE 2 (GFP-RHD2) as endosomal and apical PM marker. Specific subcellular distribution in root hair formation zone and tip-focused accumulation in growing root hairs of both markers is visualized in 2D maximum intensity projections (A, Supplemental Video S1; C, Supplemental Video S3) and 3D volumetric renderings (B, Supplemental Video S2; D, Supplemental Video S4). Bar = 50 µm (A, C). Gridline spacing = 10 µm (B, D). Imaging: (A–D) W Plan-Apochromat 20×/1.0 NA, 488 nm excitation at 2% of relative laser power level, BP505–545 detection encompassing the emission maximum of GFP (509 nm), light sheet thickness 4.52 µm, z-stacks of 0.48 µm/plane, encompassing the volume of 438.69 × 438.69 × 152.08 µm (x × y × z) in (A, B) and 438.69 × 438.69 × 229.79 µm (x × y × z) in (C, D).