Table 1.
Overview of plant volumetric LSFM imaging used in the last 3 years
| Aim | Plant, nematode and fungal species | Studied sample (cell/tissue/organ/whole plant fixed/living, sample modifications) | Imaging method | References |
|---|---|---|---|---|
| Tissue specific and developmentally regulated localization of PLDα1 (PLDα1-YFP) | Arabidopsis thaliana Col-0, pldα 1-1, pldα 1-2 | Whole root, root cap, and rhizodermis cells (live-imaging) | LSFM | Novák et al. (2018) |
| Plant–nematode interactions in the rhizosphere | Arabidopsis thaliana Nicotiana benthamiana Nicotiana tabacum Lactuca sativa Petunia multiflora Free living nematodes from soil | Whole root (live-imaging) |
|
O’Callaghan et al. (2018) |
| Plant–fungi interactions in the rhizosphere | Arabidopsis thaliana Piriformospora indica Mortierella hyaline Verticillium dahlia Alternaria brassicicola | Whole root (live-imaging) | LSFM | Meents et al. (2019) |
| Microtubule orientation during cell division (GFP-TUA6, GFP-MBD) | Arabidopsis thaliana Col-0 Medicago sativa Regen SY | Whole root, root epidermal cells (live-imaging) | LSFM | Vyplelová et al. (2018) |
| Root 3D reconstruction, cortex and epidermal cell files patterning, and root hair spacing under low phosphate availability | Arabidopsis thaliana Col-0 |
Root tip excised from plant (propidium iodide staining) |
LSFM | Janes et al. (2018) |
| Calcium oscillation in roots expressing the cytosolic-targeted FRET-based sensor Cameleon YC3.6 | Arabidopsis thaliana Col-0 | Primary root and root hairs (live-imaging) | LSFM | Armada et al. (2019) |
| Root meristematic cell division (CYCLINB1,1-GFP) | Arabidopsis thaliana Col-0 | Region of root meristem (live-imaging) | LSFM | Buckner et al. (2019, 2020) |
| Spatial distribution of lateral root primordia initiation and outgrowth in response to external water availability and in relation to the medium surface | Arabidopsis thaliana Col-0, the reporter line pLBD16::LBD16-GFP | Excised mature root segment (multiview imaging) | LSFM | von Wangenheim et al. (2020) |
| Ectopic cell division in primary and lateral roots of katanin1 mutant | Arabidopsis thaliana Col-0 and ktn1-2 expressing 35S::GFP:TUA6 | Primary and lateral roots (live-imaging) | LSFM | Ovečka et al. (2020) |
| Spatial distribution in deep tissues, developmental and subcellular localization of ANNEXIN1 (ANN1-GFP) | Arabidopsis thaliana Col-0 | Developing roots, trichoblasts, root hairs (live-imaging) |
|
Tichá et al. (2020c) |
| Plant germline differentiation and 3D modeling of flowers at subcellular level, male and female meiosis, and pollen asymmetric division (H2B-mRuby2, ASY1-eYFP, HTA10-RFP, DR5-N7-Venus, PCNA-TagRFP) | Arabidopsis thaliana Col-0, asy1, smg7-1 | Whole flowers (live-imaging) | LSFM | Valuchova et al. (2020) |
| Developmental expression pattern of FSD1-GFP during germination and early seedling development, tissue-specific localization | Arabidopsis thaliana Col-0 and fsd1-1 expressing pFSD1-FSD1::GFP:3′-UTR-FSD1 | Seed germination, seedling development, root growth, root hairs (live-imaging) | LSFM | Dvořák et al. (2021) |
| Examination of auxin reporter lines for overview of auxin distribution during flower head patterning and phyllotaxis development | Gerbera hybrida and transgenic line expressing DR5rev::3XVENUS-N7 | Entire excised flower heads, VENUS, and chlorophyll autofluorescence imaging | LSFM | Zhang et al. (2021) |
SPIM, selective plane illumination microscopy; BSPIM, biospeckle selective plane illumination microscopy, PLDα1, PHOSPHOLIPASE Dα1, GFP-TUA6, GFP-TUBULIN ALPHA 6, GFP-MBD, GFP-MICROTUBULE-BINDING DOMAIN, FSD1-GFP, IRON SUPEROXIDE DISMUTASE 1-GFP.