Fig. 3.

Schematic illustration of binding sites of M75 and eight validated antibodies. The positions of antibody binding regions are shown on the schematic model of the CA IX protein comprising a proteoglycan-like (PG) region, carbonic anhydrase (CA) domain, transmembrane (TM) anchor, and intracytoplasmic (IC) tail. Antibody arrangement reflects the information available in the datasheets. Monoclonal and polyclonal type of antibody is depicted using full and dashed line, respectively. No information regarding the immunogen used for the generation of 2D3 monoclonal antibody is available in its datasheet. According to “Ten basic rules of antibody validation” [101], all selected antibodies are correctly described by providers and, with exception of 2D3, have a defined immunogen