Figure 5.

Experimental setup designed for time-lapse analysis of endodermis nuclei within the TAD of the RAM. A, Nikon AZ100 Multi Zoom with epifluorescence supported in horizontal position. Plants were maintained in homemade chambers prepared within a Petri dish (see “Materials and methods”), and roots grew on a surface of agar growth medium. Environmental light and temperature conditions were controlled to ensure growth conditions similar to A. thaliana growth room environments. Chambers were positioned in front of the microscope lenses and supported by automated translation stage base plates to control X, Y, and Z positions of the specimen. This setup allowed roots to grow parallel to the gravity axis. B, The Multi Zoom system permitted alternating between magnifications to capture either images of the root apex as in (A), or images of individual nuclei, as in (B). Scale bar = 20 μm. These images were taken in the Laboratorio de Microscopía y Microdisección Láser (LabMicroLas) at the Instituto de Ecología, UNAM, Mexico.