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. 2021 Oct 23;188(2):816–830. doi: 10.1093/plphys/kiab490

Figure 7.

Figure 7

Automatic segmentation applied to different plant species. Top line: poplar stem bark fibers, middle line: flax stem xylem, bottom line: maize stem vascular bundle. First column: merged CLSM image of green, red, and magenta fluorescence channels, second to fourth columns: cell wall zone segmentation for CC, CML, and SCW. All samples were labeled by the triple lignin bioorthogonal chemical reporter strategy with H*, G*, and S* monolignol reporters. Square frames indicate the sample region magnified (bottom RHC, Columns 2–4). Scale bar main image = 20 µm, scale bar insert = 5 µm.