Figure 4.

Increased global nuclear pore density in patient fibroblasts. (B) Compressed Z-stack representative immunostained images of mAB414 (green spots) and nucleus (DAPI; blue) in patient (P) fibroblasts and controls. (C) Quantification of mAB414 immunostained NPC pore density (pores per μm² of nuclear surface area) in patient fibroblasts compared to controls (C1—C4) shows a significant increase in NPC pore density in patient fibroblasts compared to three of the four controls. N = 3 biological samples/cell line, pooled data from 40–79 nuclei/cell line. One-way ANOVA with Sidak’s multiple comparisons test; ** P < 0.01, ^***P < 0.001. (D) Distance to the nearest mAB414 immunostained pore neighbour measurement shows a significant decrease in distance in patient fibroblasts compared to controls, indicating more densely populated NPCs in patient fibroblasts. N = 3 biological samples/cell line, pooled data from 40–79 nuclei/cell line. One-way ANOVA with Sidak’s multiple comparisons test; ^***P < 0.001.