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. 2021 Nov 19;188(2):861–878. doi: 10.1093/plphys/kiab537

Figure 2.

Figure 2

Single-cell transcriptome of A. thaliana above-ground tissue. A, t-SNE projection plot of 37 clusters identified from 18,313 cells in Col-0 rosette tissues harvested at the ED (n = 3 replicates). B, t-SNE visualization of 25 clusters identified from 31,665 cells in Col-0 rosette tissues harvested at the EN (n = 3 replicates). C, t-SNE projection plot showing 16 main clusters identified from 49,978 pooled cells in Col-0 rosette tissues harvested ED and EN (n = 6 replicates). Each dot represents the transcriptome from one cell. Cells represented by the same colors correspond to the same cluster. Cells identified in different clusters but belonging to the same tissue types are represented with similar colors. The cluster robustness was scored based on cell co-occurrence in subsampled data 75%–100% (***), 50%–75% (**), 25%–50% (*) (see “Materials and methods”; Supplemental Figure S7). D, Schematic representation of rosette cell types of Arabidopsis plants. Upper panel represents longitudinal section through the meristem, whereas lower panel shows longitudinal and cross section of the leaf.