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. 2022 Jan 26;11:739628. doi: 10.3389/fcimb.2021.739628

Figure 3.

Figure 3

Kinetic measurement of the signal of EV RNA staining Area threshold inside recipient monocytes or macrophages. Pf-derived EVs were labeled by Thiazole orange (TO), and their uptake into monocytes and macrophages was measured for 1 hour. (A) Schematic representation of the Area Threshold feature. (B) Graph representing change in area threshold of the fluorescent signal inside the recipient cells over time. Cell types were compared with a linear mixed effects model, with cell type and time as fixed factors, and sample ID as a random factor. Statistics were done in R, v. 4.0.4, using the package ‘lmerTest’. For calculating changes relative to the starting point, a log2-fold change was calculated per each point, relative to its own sample’s average value of the first 1.5 minutes. Grey area around the lines represents 95% confidence intervals, p<0.001. (C) Signal detected by IFC from three representative recipient cells of monocytes and macrophages at the first 10 minutes of the uptake. (D) Signal detected by IFC from three representative recipient cells of monocytes and macrophages at 1 hour post uptake. BF-bright field, TO-thiazole orange, Hoechst-nuclear dye.

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