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Puerarin decreased the mRNA expression of the TLR4, MyD88, and TRAF6, in HRV Wa-infected Caco-2 cells. The mRNA expression of the TLR4, MyD88, and TRAF6 in Caco-2 cells was detected by RT-qPCR analysis (A-C). Significant differences between groups were indicated as ##, P<0.01 vs. NC group; *, P<0.05; **, P<0.01 vs. RV group, n=3. TLR4, Toll-like receptor 4; HRV Wa, human RV G1P[8] Wa strain; RT-qPCR, real-time reverse transcription PCR; NC, normal control; RV, rotavirus.
