Table 1. Primer sequence for RT-qPCR.
Target | Forward (5´ to 3´) | Reverse (5´ to 3´) |
---|---|---|
TLR4 | AGGTTTCCATAAAAGCCGAAAG | CAATGAAGATGATACCAGCACG |
MyD88 | CGCCGCCTGTCTCTGTTCTTG | GGTCCGCTTGTGTCTCCAGTTG |
TRAF6 | GCAGAGAAGGCGGAAGCAGTG | TGGTAGAGGACGGACACAGACAC |
β-actin | CCCATCTATGAGGGTTACGC | TTTAATGTCACGCACGATTTC |
RT-qPCR, real-time reverse transcription PCR; TLR4, Toll-like receptor 4.