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. 2022 Jan 24;12(4):1738–1755. doi: 10.7150/thno.64148

Figure 6.

Figure 6

CCZ1-MON1A regulates APP-CTFs and Aβ levels through autophagy. (A) N2S cells were transfected with Flag-CCZ1/Flag-MON1A in the presence or absence of SAR405 and CQ. The levels of FL-APP and APP-CTFs were examined by immunoblotting. (B-C) Quantitative results of Figure 6A show that SAR405 and CQ blocked the CCZ1-MON1A over-expression-mediated reduction of APP-CTFs. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the relative control. (D-E) CCZ1-MON1A over-expressing N2S cells were treated with SAR405 or CQ, and the intracellular Aβ1-40 and Aβ1-42 levels were examined by ELISA. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the relative control. (F-H) N2S cells were transfected with control or Atg5 siRNA 48h before being transfected with CCZ1-MON1A plasmids. FL-APP and APP-CTFs levels were determined by immunoblotting. Data are quantified as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, vs. the relative control. (I-K) N2S cells were transfected with control or Atg7 siRNA 48h before being transfected with CCZ1-MON1A plasmids. APP-CTFs levels were determined by immunoblotting. Data are quantified as mean ± SEM (n = 3). *P < 0.05, **P < 0.01 vs. the relative control.