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. 2022 Jan 24;12(4):1738–1755. doi: 10.7150/thno.64148

Figure 8.

Figure 8

AAV-mediated CCZ1-MON1A over-expression in hippocampus alleviated autophagy impairment and AD related neuropathological changes in 3xTg AD mice. (A-G) 2 months after control AAV or CCZ1-MON1A AAV injection, the hippocampus tissues of mice were dissected and subjected to immunoblotting analysis of LC3-II, SQSTM1, FL-APP and APP-CTFs. Data are quantified as mean ± SEM (n = 9). *P < 0.05, **P < 0.01, vs. the relative control. (H) The mice brain sections were stained with 4G8 antibody to visualize the Aβ plaques and intraneuronal Aβ accumulation. Bar: 200 μm. (I-J) Intracellular Aβ1-40 and Aβ1-42 in mice hippocampus were measured by ELISA. Data are quantified as mean ± SEM (n = 9). *P < 0.05, **P < 0.01, vs. the relative control. (K-N) The phosphorylated tau in mice hippocampal lysates were determined by immunoblotting using antibodies indicated on the figure. Data are quantified as mean ± SEM (n = 9). *P < 0.05, **P < 0.01 vs. the relative control.