Figure 3.

Exosomes delivered by tail vein injection were mainly taken up by hepatic macrophages in mice with CCl₄-induced fibrosis. Exosomes were stained with Dil and approximately 200 µg (protein equivalent) of exosomes in 150 µL of PBS were injected into mice with CCl₄-induced fibrosis via the tail vein. (A) After 6 h, Dil signals in the liver, lung, spleen, kidney, and heart were examined using bioluminescence imaging. After 6 (B) or 48 h (C) of injection of Dil-labeled exosomes, liver sections were stained with an anti-mouse F4/80 antibody and analyzed by fluorescence microscopy. Nuclei were counterstained with Hoechst 33258.