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. 2022 Jan 1;12(4):1486–1499. doi: 10.7150/thno.66119

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PDL1-dependent CD40 agonism of CD40/PDL1-bispecific antibody variants. (A, B, C) HT1080-CD40 cells were stimulated in the presence of HEK293 cells transfected with empty vector (EV) or an PDL1-encoding expression plasmid with the indicated concentrations of αCD40-Fab₂-HC:scFvPDL1 and αCD40-Fab₂ (A), αCD40-IgG1(N297A)-HC:scFvPDL1 and αCD40-IgG1(N297A) (B) or αPDL1-IgG1(N297A)-HC:scFvCD40 and αPDL1-IgG1(N297A) (C). Next day, CD40 activation was quantified by measuring upregulation of IL8 production by ELISA. Shown are data obtained by three independent experiments.