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T-cell costimulation by a CD19-BiTe and α41BB-IgG1(N297A)-HC:scFvPDL1. K562 cells and K562-CD19 transfectants were seeded in 96-well plates (35 x 103 cells/well). Next day, cells were challenged as indicated with PBMCs, Empty vector or PDL1 transfected HEK293 cells and α41BB-IgG1(N297A)-HC:scFvPDL1. After an additional day IL2 production was analyzed by ELISA. Shown are the averaged results of 6 independent experiments.
