Table 1.
Binding affinities of investigated aCD40/PDL1-antibody fusion proteins. For statistical analysis see supplemental data Figure S1.
| Antibody Fusion Protein | Interaction | Number of Experiments | Single Kd (ng/mL) | Mean Kd (ng/mL) |
|---|---|---|---|---|
| αCD40-Fab2-GpL | CD40 | 3 | 830, 600, 780 | 740 ± 120 |
| PDL1 | 2 | n.b. | - | |
| αCD40-Fab2-HC:scFvPDL1-GpL | CD40 | 3 | 890, 600, 1260 | 920 ± 330 |
| PDL1 | 4 | 190, 90, 140, 250 | 170 ± 70 | |
| αCD40-N297A-GpL | CD40 | 3 | 1340, 430, 990 | 920 ± 460 |
| PDL1 | 2 | n.b. | - | |
| αCD40-N297A-HC:scFvPDL1-GpL | CD40 | 3 | 1490, 730, 1080 | 1100 ± 380 |
| PDL1 | 4 | 290, 290, 300, 240 | 280 ± 30 | |
| αPDL1-N297A-GpL | CD40 | 2 | n.b. | - |
| PDL1 | 6 | 220, 210, 130, 270, 300, 190 | 220 ± 60 | |
| αPDL1-N297A-HC:scFvCD40-GpL | CD40 | 3 | 1800, 1210, 1520 | 1510 ± 300 |
| PDL1 | 4 | 150, 80, 260, 270 | 190 ± 90 |
n.b.; no binding