Fig 1.

Glial cell production of βHB and its regulation by GKRP. A. Lactate production in tanycytes incubated at different glucose concentrations for 1 h. B-C. Western blot and quantification for phosphorylated AMPK (pAMPK), total AMPK (AMPK), MCT1 and β-actin loading control from the total protein of tanycytes after stimulating with glucose as described in A. D. βHB production in astrocytes and tanycytes primary cultures upon AMPK activity activation with 0.5mM Aicar or AMPK inactivation with 15µM compound C (CpC). E. βHB production in cortical astrocytes and tanycytes in response to different glucose concentrations for 1 h.