Fig. 7.
Post-isolation of exosome standards spiked into various matrices (50 μL) using the PET C-CP fiber tip spin-down method; the concentration of exosomes recovered were determined based on ELISA readout to an exosome standard curve of linear response was performed to quantitatively detect the expression of the exosomal tetraspanin protein-CD81 (n = 3) employing a capture antibody of 1:250 concentration. Quantified recovery of exosomes from mock matrices of 1/100 and 1/1000 concentration. The loaded 1/100 and 1/1000 solutions theoretically contain 2 × 1010 and 2 × 109 exosomes, respectively