FIGURE 6.

Decrease in IL‐1β‐induced expression levels of IL‐8 and IL‐6 in FHs 74 Int cells by SCFAs. FHs 74 Int cells were pretreated with or without acetate, propionate, or butyrate (a, 5−30 mM, b, 20–30 mM, c–f, 20 mM) for 1 h, and then incubated for 24 h with IL‐1β (0.5 ng/ml). IL‐8 (a) and IL‐6 (b) concentrations in cell cultures supernatant were determined by ELISA kit Sensitivity of cell viability (c) and cytotoxicity (d) to SCFAs was detected by CCK‐8 and LDH assay. IL‐8 mRNA (e) and IL‐6 mRNA (f) levels were evaluated by qRT‐PCR. Data are represented as mean ± SD. Data with different letters indicated significant differences (p < .05) between groups