Table 3.
SV results for phage CBA120 TSPs.
| Protein | Oligomeric form | MWcalc (kDa)a | S20,w (S)b | MWaap (kDa)c | f/f0b |
|---|---|---|---|---|---|
| TSP1 | Trimer | 248 | 9.6 | 276 | 1.55 |
| TSP114-166 | Trimer | 50 | 3.6 | 53 | 1.43 |
| TSP2 | Trimer | 297 | 9.9 | 279 | 1.56 |
| TSP3 | Trimer | 206 | 8.4 | 195 | 1.43 |
| Hexamer | 412 | 12.9 | 357 | ||
| TSP3156-627 | Trimer | 180 | 7.7 | 140 | 1.25 |
| Hexamer | 360 | 12.9 | 300 | ||
| Nanomer | 540 | 17.7 | 490 | ||
| TSP4-N335 | Monomer | 36 | 2.7 | 39 | 1.46d |
| Trimer | 109 | 5.2 | 86 | 1.33d | |
| Hexamer | 218 | 7.7 | 199 | 1.67d | |
| TSP4-N490 | Monomer | 53 | 3.1 | 39 | 1.58 |
| Hexamer | 316 | 8.8 | 264 | 1.69 | |
| TSP4-N181 | Hexamer | 120 | 6.1 | 109 | 1.45 |
| TSP4-N253 | Hexamer | 167 | 6.9 | 125 | 1.36 |
| TSP4185-1036 | Trimer | 273 | 9.8 | 201 | 1.27 |
aMolar mass calculated from the amino acid composition of the proteins.
bS20,w and best fitted frictional ratio (f/f0) values from the SEDFIT analyses using the continuous c(s) distribution model.
cExperimental molar mass distribution c(M) values from the SEDFIT analyses using the continuous c(s) distribution model. Note that the c(M) values are correlated to the f/fo values, which are subjected to the number and shapes of species present in the sample.
dTSP4-N335 monomers and hexamers are purified as single species. Trimeric TSP4-N335 exists in the presence of monomeric and hexameric species.