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. 2022 Feb 8;13(2):127. doi: 10.1038/s41419-022-04586-2

Fig. 4. Expression of inflammatory mediators triggered by LPS-induced sepsis was diminished in the presence of a mitoNEET inhibitor.

Fig. 4

C57BL/6 mice were injected intraperitoneally with vehicle, LPS (20 mg/kg), or LPS plus NL-1 (20 mg/kg). Blood was collected from the right atrium 48 h later, and IL-6 and TNFα levels in serum were measured (A and B). Total RNA was harvested from the spleen and liver. Expression of mRNA encoding iNOS and COX2 was assessed in the spleen or liver (C and D) by quantitative real-time RT-PCR. For all real-time PCR analyses, β-actin was used as a control for normalization. All data are expressed as the mean ± SD from three independent experiments. *P < 0.05 for LPS vs. LPS plus NL-1 treatment.