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. 2022 Feb 8;13(2):127. doi: 10.1038/s41419-022-04586-2

Fig. 7. Inhibition of mitoNEET increases expression of antioxidant defense genes.

Fig. 7

Control shRNA-expressing or mitoNEET-expressing RAW264.7 cells were treated with vehicle, LPS (1 µg/mL), or LPS plus NL-1 (20 µM). Total RNA and protein were harvested 12 h later, followed by measurement of mRNA encoding HO-1, SOD2, or SOD1 by quantitative real-time RT-PCR (A–C and E–G). Expression of HO-1, SOD2, and SOD1 protein was detected by western blotting (D and H). All data are expressed as the mean ± SD from three independent experiments. *P < 0.05 for LPS vs. LPS plus NL-1 treatment. †P < 0.05 for control shRNA-expressing cells vs. mitoNEET shRNA-expressing cells in the presence of LPS. NS, not significant.