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. 2022 Feb 8;13:750. doi: 10.1038/s41467-022-28358-w

Fig. 4. Mitochondrial DNA editing in neonatal mouse hearts.

Fig. 4

a Scheme of in vivo experiments with neonatal mice. The DdCBE-Nd3-9577-1 monomers (see Fig. 2), and their catalytically inactive versions, were encoded in separate AAV genomes, encapsidated in AAV9.45 then simultaneously administered by temporal vein injection at 1 × 1012 vg/mouse of each monomer. Animals were sacrificed 3-weeks post-injection and their cardiac tissue was examined for mtDNA editing. b Editing of mouse MT-Nd3 with DdCBE in neonatal mouse heart at 3-weeks post-injection, analyzed by Sanger sequencing. Potential editing sites are indicated in purple. c The NGS analysis of the DdCBE editing within the targeted region in neonatal mouse hearts. Bars represent the mean and error bars represent ±SEM (n = 7). Source data are provided as a Source Data file. d The distribution of NGS reads containing m.9576 G (C12) or m.9577 G (C13) edits in neonatal hearts at 3-weeks post-injection. The G40K reads contain both m.9576 G > A (C12 > T12) and m.9577 G > A (C13 > T13) mutations, G40E reads contain only the m.9577 G > A (C13 > T13) mutation, while G40* reads contain only the m.9576 G > A (C12 > T12) mutation. Source data are provided as a Source Data file.