Figure 4.

K3-SPG ISV induces immunological memory. KPC-N-bearing mice were treated with PBS or K3-SPG-ISV (10 μg) on days 7, 9, 11, 13, and 15 after tumor inoculation (n = 5). All the K3-SPG-ISV-treated mice were cured. On day 56, the cured mice were rechallenged with the second round of subcutaneous inoculation of 2 × 10⁶ KPC-N and age-matched naïve C57BL6 mice (n = 5) were subcutaneously inoculated with the same number of KPC-N as a control cohort. All cured mice rejected rechallenge with KPC-N. On day 162, they were subcutaneously challenged with 2 × 10⁶ MC38 cells. The tumor growth curves over the entire time course are presented. Data are representative of two independent experiments with similar protocols and results. The arrows indicate the timing of therapy. Error bars represent the mean ± SEM. Statistically significant differences were measured by one-way ANOVA with Dunnett’s post hoc test. *p < 0.05.