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. 2022 Feb 8;12:2067. doi: 10.1038/s41598-022-06118-6

Figure 1.

Figure 1

Non-injected and microinjected zygote development rates to the blastocyst stage and mutation efficiencies following microinjection of Cas9 protein and in vitro transcribed (IVT) test gRNAs targeting the 5′ and 3′ regions flanking the 10 bp on the polled locus at 18 h post insemination. (a) Blastocyst development rate of microinjected and non-injected control embryos (green) when targeting the 5′ and (b) 3′ regions flanking the 10 bp target region. (c) Mutation rates in embryos injected with Cas9 and gRNAs targeting the 5′ and (d) 3′ regions flanking the 10 bp target region. Error bars = SEM. **P < 0.01.