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. 2022 Feb 8;12:2067. doi: 10.1038/s41598-022-06118-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Histological analysis of gene edited fetuses along with polled and horned controls. (a,d,g) Horn bud region of aged matched polled and (b,e,h) horned control fetuses alongside representative fetuses from (c) ETs 1, (f) 2 and (i) 4. Multiple layers of vacuolated keratinocytes can be seen in the horn bud region of horned fetuses along with sebaceous glands (black arrows) and nerve bundles (black stars). Stained with Hematoxylin and eosin. gd = gestation days.