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. 2022 Jan 26;9:825257. doi: 10.3389/fvets.2022.825257

Figure 2.

Figure 2

Preparation of plasmid standards: (A–E): amplification curves (X-axis: Cycle, Y-axis: ΔRn) of BVDV, BPIV3, BRSV, BoHV-1 and IDV for each plasmid standard with concentrations ranging from 1 ×107copies/μL to 1 × 101copies/μL; (F–H): standard curves of plasmid standards of BVDV, BPIV3, BRSV, BoHV-1and IDV. (F): BVDV: Y = −3.125log(X)+39.474 R2 = 0.999 Eff% = 108.905; (G): BoHV-1: Y = −3.312log(X)+38.736 R2 = 0.992 Eff% = 100.424; (H): BPIV3: Y = −3.206log(X)+38.173 R2 = 0.999 Eff% = 105.053; I: BRSV: Y = −3.322log(X)+39.273 R2 = 0.995 Eff% = 100. 104; (J): IDV: Y = −3.165 log(X)+38.044 R2 = 0.99 Eff% =107.012.