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Specificity of the multiplex real-time PCR assay: The data were represented with amplification curves (X-axis: Cycle, Y-axis: ΔRn) (A–E) Amplification curves represent samples positive for BVDV, BoHV-1, BPIV3, BRSV and IDV detected by the multiplex real-time PCR assay. (F) Co-infection simulation experiments with nucleic acids of five pathogens extracted from cell culture The viruses can be detected by our multiplex real-time PCR.
