Table 2.
Primers used for standard constructions.
| Pathogens | Primer or probes | Sequence (5'-3') | Position | Length |
|---|---|---|---|---|
| BVDV | BVDV-F54 | CCGGAATTCCGGGACAAATCCTCCTTAGCGAA(EcoRI) | 64–85 | 450 bp |
| BVDV-2F54 | CGC ctcgag TCTCCTCTCTCGCCAAACA(XhoI) | 476–494 | ||
| BOHV-1 | BOHV-1-gI-gE-L | gttaTCTAGAgtgatggtgatggtgatgGCGGAGGATGGACTTGAGTCG(xba I) | 120290–120309 | 3188 bp |
| BOHV-1-g3E-U | GATC gactagt CGGTGCCTGTTGCTCTGGAT(Spel I) | 123418–123438 | ||
| BRSV | BRSV-NF2 | GATCGACTAGTC GCCACCATG ATGTTATATGCTATGTCCCGAT (Spel I) | 200–221(N) | 980 bp |
| BRSV-NR2 | GTTA CTCGAG CCAATTGGTTCTTGATTGCCTC (Xhol) | 1127–1147(N) | ||
| BPIV3 | BPIV3 MF1 | CTAG TCTAGAAACGAACAAAGGAAGGCAAT(XbaI) | 3834-3853 | 881 bp |
| BPIV3 MR1 | CGC GGATCCATGATGCCCATATAACCAGA (BamHI) | 4676–4695 | ||
| IDV | IDV-F | GATCGACTAGTCGCCACCATG acgtcaatgatatcattgaca(Spel I) | 68–88 | 2135 bp |
| IDV-R | GTTACTCGAGC ttcaattgcctctcccatcga (Xhol) | 2150–2170 |