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. 2022 Feb 8;9:8. doi: 10.1186/s40580-022-00299-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Morphology of the Caco-2 epithelial cells cultured in a static culture condition and in the gut-on-a-chip with microfluidic flow for 5 days. A and D are phase contrast views of Caco-2 cells monolayer. B and E show the distribution of the cytoskeleton protein, F-actin, in the Caco-2 cells monolayers. C and F show the distribution of the tight junction protein, ZO-1, in the Caco-2 cells monolayers. Scale bars are 25 μm